| Milla Jovovich (Alice Prospero) |
| Sienna Guillory (Jill Valentine) |
| Oded Fehr (Carlos Olivieira) |
| Thomas Kretschmann (Le major Cain) |
| Jared Harris (Le Dr Charles Ashford) |
| Sandrine Holt (Terri Morales) |
| Sophie Vavasseur (Angie Ashford) |
| Mike Epps (L. J.) |
| Jason Isaacs (William Birkin) |
| Emily Bergl (Claire Redfield) |
| Zack Ward (Nicholai Sokolov) |
| Razaaq Adoti (Peyton Wells) |
| Matthew G. Taylor (Nemesis) |
| Stefan Hayes (Yuri Loginova) |
| Iain Glen (Le docteur Isaacs) |
| Geoffrey Pounsett (Mackenzie) |